PacBio HiFi whole genome sequencing of adult male Leptoglossus phyllo... - SRA

SRX23987820: PacBio HiFi whole genome sequencing of adult male Leptoglossus phyllopus UT Arlington
1 PACBIO_SMRT (Sequel IIe) run: 10.5M spots, 128G bases, 44.4Gb downloads

Design: High molecular weight (HMW) DNA extraction was performed from the using the Qiagen MagAttract HMW DNA Kit. DNA shearing was performed using the Diagenode Megaruptor 2 with the 20 kb fragment protocol. The sheared DNA was prepared for PacBio sequencing using the SMRTBell Express Template kit 2.0. The library was size-selected with beads to remove library molecules less than 3 kb in length to generate the final libraries for sequencing. Sequencing was performed on a Sequel II System using Binding Kit v2.0, Sequencing kit v2.0, and SMRT Cell 8M. To target HiFi reads, the library was sequenced using a 30 hour movie time on one SMRTcell. Raw subreads were converted to HiFi data by processing with CCS to call a single high quality consensus sequence for each molecule, using a 99.5% consensus accuracy cutoff.

Submitted by: United States Department of Agriculture

Study: Genome sequencing of Leptoglossus phyllopus

PRJNA1089323 • SRP496266 • All experiments • All runs

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The purpose of this project is to sequence and assemble the genome of one female and one male Leptoglossus phyllopus, the Eastern leaf-footed bug, a widespread and conspicuous minor pest of many fruits, vegetables, grains, nuts and ornamentals.

Sample: Leptoglossus phyllopus male HiFi/HiC

SAMN40534740 • SRS20785296 • All experiments • All runs

Organism: Leptoglossus phyllopus

Library:

Name: ihLepPhyl2_male_PacBio_CCS_low

Instrument: Sequel IIe

Strategy: WGS

Source: GENOMIC

Selection: RANDOM

Layout: SINGLE

Runs: 1 run, 10.5M spots, 128G bases, 44.4Gb

Run	# of Spots	# of Bases	Size	Published
SRR28382979	10,495,019	128G	44.4Gb	2024-03-19

ID:: 32293750

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